Your analysis makes sense. If you're not calling pre with AJo, you don't have enough Jacks in your range. So TT is the next best hand.

Are microstakes players bluffing enough? Don't know because only you know your player pool best. My impression is that enough people will bluff AK/AQ/KQ in this spot to make bluffcatching profitable.

However, for some people 3bet pre/bet/bet/jam is an extremely nutted line, so beware. If you had a read on the player as a passive player, or a standard multitabling reg, I would definitely fold here. Against unknowns, just play fundamentally sound.

Just a point about terminology. When you calculate how much of your range you need to defend, you're probably using Minimum Defence Frequency. MDF is a fine concept in many cases, but MDF and GTO aren't the same thing. See this post of mine for an example.

Hi, I think that you have analysed the hand really well and everything you wrote sounds good to me, although I have not worked out the combos myself it all sounds reasonable.
I think that as a general rule of thumb at 25nl which is the stake I play too, that villains do not triple enough as bluffs in the majority of situations, but this is all very player dependent and you have a note now that this villain is capable of doing so.
What I do notice is that I see more triple brl bluffs from PF aggressors when they are OOP. I also have noticed that players will squeeze wider than they will 3b in a lot of cases. I am surprised that villain goes 3 streets here given your super snug range. I think that a lot of players in your situation would even fold some AJ & KQ combos, and so if i were the squeezer here I would say you have a lot of 99-JJ as a big chunk of your range and would probably give up river given the run out. Especially when he blocks AK & AQ.
I think this is more likely spew than anything, his flush card blocker is not super relevant as the flush draw is not present on the flop so less likely you start calling to hit the flush, way more likely you have a hand exactly like you do.
In terms of adjusting, i would fold more of my range on the flop and turn, but not by a huge amount, and not the medium pocket pair portion of the range but perhaps the weaker Ax and KQ hands.

What to call against squeeze?
That is highly depend on the specific player but as you have no info on villain I would look at the player pool in most player pools you see that the looser guys tend to squeeze wider then they 3 bet. The good regs will most of the time not squeeze light as there is an UTG player involved. On the SB I would assume that most players tend to be more linear 3 betting and don't have a calling range. So would say that they are heavy in broadway cards such as AK,AQ,AJ,KQ,QJ both suited and none suited as well as TT+ and JTs (total 8.6%) or even tighter due to UTG opening. So I think the range you specified is ok-ish against John Doe.

Flop
Against a 33% bet and being IP you can defend very wide you could even call with just all hands.

Turn
The Jack hit both ranges equally that there is a flush-draw out there is of little importance as it wasn't there on the flop. Here I normally want to know more on my opponent before I continue with a pair below the J. But still I would most of the time continue on the turn with TT you don't block overcards and he can have a lot and his 33% flop bet did not mean much so villain still have loads of bluff cards.

River
The flush hits that is not a good card on one hand on the other hand it is a card that some players like to bluff on and some other players might check turn with a flush or be scared of the flush card and check to you with an overpair. So in fact the card is not to bad. But having said that the river is heavily under bluffed in a lot of player pools. So this is a close EV spot in my experience.

All in all I think you analyzed it very well from a theory perspective, but should look also at the player pool to check if there are enough players that bluff enough at the river or that that the player pool consists of mostly NITS as that can make the difference between a +EV call or a -EV call here.

I will agree on using MDF basically only on the river(other streets less important). Ranges are more important in most cases, if villain range is too tight and board is where we can't realize our Equity we would fold much more than MDF.

Few examples:
4bet spot Axx and we OOP, vs 1/4 size we might fold up to 50% of our range
3bet spot Kxx CO/MP vs 1/3 size range bet, we would fold roughly 40% on the flop because hard to realize equity.
SRP BB/MP something like AA2 (again a lot of folds even vs smallish size)

Can see pattern here, that it is unprofitable to play OOP many hands. But even IP vs sqz range, villain in many situations just can bet flop range(that's reason why we calling because many hands just have too much equity vs range bet), barrel turn a lot and pick up bluffs on the river. And depending how our range construct we might overfold flop/ and some turns but river nopeee.

Anyway about the hand, I think it is close to 0 EV bluffcacther, would prefer having Tc

Nice call down. Is this squeeze size standard in your player pool?
Also what'd he have?